Assessment of Time Periods of Exogenous Development of Trichostrongylus tenuis Nematodes, Parasitizing in Domestic Goose

Abstract

The aim of present study was to assess the time periods of exogenous development of Trichostrongylus tenuis nematodes that parasitize in domestic goose, and their survival in vitro. To do that, eggs of Trichostrongylus were obtained from excrement of infected birds and cultured in laboratory to the third stage larvae (L3). Culturing was performed at 15, 20 and 25 °C. Time periods of the parasitic development was analyzed, as well as their survival. According to results, the optimum temperature of embryonic and postembryonic development of T.tenuis is 25 °C. The time period of exogenous development at 25 °C was the shortest (five days), and survival was 83.67%. Decreasing temperature resulted in slower development of infectious larvae (10 days) of lower survival (58.67%). Developmental processes in eggs and larvae of Trichostrongylus were accompanied by changes in their sizes regardless of the temperature conditions. Parasitic embryogenesis was characterized by increasing length and width of eggs. Postembryonic development of nematodes was associated with inflating length and width of second stage larvae, but inflating length and decreasing width of third stage larvae.